Short Chain Fatty Acids Targeted Panel
Rigorous Quality Control
About Short Chain Fatty Acids
Metabolomics reveals biological insights otherwise unseen. For a successful metabolomics study, both small molecule discovery and the ability to dig deeper into specific biomarkers of interest are needed to uncover actionable insights that propel new therapeutic developments. A specific combination of liquid chromatography-mass spectrometry (LC-MS) technology and expertise is required to identify these biomarkers of interest and develop assays that are sensitive enough to explore them fully.
At Metabolon, we understand the crucial role SFCAs play in gastrointestinal disorders, and we’ve established best-in-class expertise. This panel focuses on specific SFCAs and their metabolic pathways and can be used to track biomarkers and enhance biological understanding across preclinical and clinical research.
Short Chain Fatty Acids Targeted Panel Details
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|Metabolite||Plasma/Serumb||Fecesb||Feces in OMNImet™·GUT tubec (µg/g dry feces)|
|Acetic Acid (C2)||1000 ng/mL||50.0 µg/g||1000 µg/g|
|Propionic Acid (C3)||100 ng/mL||25.0 µg/g||50 µg/g|
|Butyric Acid (C4)||25.0 ng/mL||25.0 µg/g||60 µg/g|
|Isobutyric Acid (C4)||25.0 ng/mL||5.00 µg/g||6.0 µg/g|
|Valeric Acid (C5)||25.0 ng/mL||5.00 µg/g||6.0 µg/g|
|Isovaleric Acid (C5)||25.0 ng/mL||5.00 µg/g||6.0 µg/g|
|2-Methylbutyric Acid (C5)||25.0 ng/mL||5.00 ug/g||5.0 µg/g|
|Caproic Acid (C6)||50.0 ng/mL||1.00 µg/g||2.0 µg/g|
|Lactic Acid||(upon request)d||6.0 µg/g|
bAssays with this sample type are for non-GxP testing and are not for diagnostic use.
cGood Clinical Practice (GCP) Assays. Lower Limit of Quantitation (LLOQ) stated is based on normalization using an average dry weight of feces. The actual measurement of the assay is the concentration of analyte in the OMNImet™·GUT tube containing human feces. Analyte concentrations in the OMNImet™·GUT tubes will be normalized by measured dry weight of feces unless otherwise requested.
dAvailable in select sample types only.
Analysis Method and Instrumentation:
LC-MS/MS (Agilent 1290 UHPLC/Sciex QTrap 5500)
|Sample Type and Required Amounts|
|Sample Type||Sample Requirement|
|Feces (wet weight)||200 to 500 mg|
|Plasma/Serum||100 to 150 µL|
|Feces in OMNImet™·GUT tube||Approx. 500 mg in OMNImet™·GUT tube, filled according to manufacturer specifications|
Disclaimer: Non-GxP assays are for Research Use Only and are not to be used for diagnostic purposes.
Delivering Absolute Quantification for Research and Biomarker Analysis
Our readily available or custom developed quantitative assays help you achieve your research and biomarker validation objectives with precise and fully validated methods. Our targeted assays and panels cover >1,000 metabolites and lipids across a wide range of biochemical classes, metabolic pathways, and physiological processes, and they can be customized to best fit any application.
Short Chain Fatty Acids Targeted Panel Applications
Recognized for our cutting-edge approach to metabolomics, Metabolon has been a valued resource for COVID-19 researchers worldwide. Our actionable metabolomic insights have fueled pivotal and high-profile studies like the National Institute of Allergy and Infectious Diseases (NIAID) IMPACC study research to improve understanding of high-risk patients and the Institute for Systems Biology research to improve understanding of high-risk patients. These and other metabolomics projects with actionable insights at Metabolon are helping get closer to the phenotype and pressing forward on COVID-19 answers.
Big Insights with Metabolon
Cited in over 3,000 publications, we help scientists and manufacturers gain greater insight into their studies through metabolomics. See how our approach can become a successful part of your workflow.
Related Metabolomics Resources
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Research Triangle Park, NC 27709
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